The refugees had departed from the western port city of Hodeidah en route to Sudan when the gunship opened fire on Friday, the United Nations refugee agency said.
The Red Sea port near the Bab al-Mandab strait is under the control of Yemen’s armed Houthi movement, which has been fighting Saudi Arabia and its allies in a two-year-old conflict.
While the Arab alliance denied responsibility for the attack on Friday, it called for jurisdiction over Hodeidah port to be transferred to the U.N.
“This would facilitate the flow of humanitarian supplies to the Yemeni people, while at the same time ending the use of the port for weapons smuggling and people trafficking,” it said in a statement. It did not address a call by Somalia to investigate.
Hodeidah is part of a broad battlefront where forces loyal to Yemeni President Abd-Rabbu Mansour Hadi, backed by the Saudi-led coalition, are fighting the Iran-allied Houthi movement which controls most of north and western Yemen.
The Saudi-led coalition was formed in 2015 to fight the Houthis and troops loyal to former president Ali Abdullah Saleh who have fired missiles into neighboring Saudi Arabia.
The Bab al-Mandab is a strategic waterway through which nearly 4 million barrels of oil are shipped daily.